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J Bacteriol. 1972 January; 109(1): 89-95
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Nutritional Studies of Rickettsia quintana: Nature of the Hematin Requirement

¹ Department of Microbiology, University of Maryland School of Medicine, Baltimore, Maryland 21201

ABSTRACT

Rickettsia quintana grew in a liquid medium consisting of a brain-heart infusion base supplemented with starch and hematin. The growth requirement for hematin could not be substituted by compounds of known catalytic activity for H₂O₂, viz., catalase, potassium pyruvate, or charcoal, or by the reducing compounds sodium sulfite and sodium thioglycollate. R. quintana was catalase-negative, but no H₂O₂ production could be demonstrated by the catalase-aminotriazole technique. A minimum inoculum giving 10⁵ cells/ml was required to initiate growth. The generation time at 33 C was 10 hr. The temperature range for growth was 28 to 37 C. Growth was enhanced when succinate or glutamate was added as energy source.

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