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Replication of the R Factor Rts1 in Proteus mirabilis

^a Laboratory of Molecular Biology and Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

The replication of the R factor Rts1 in Proteus mirabilis was examined by using the technique of CsCl density-gradient centrifugation. The proportion of Rts1 deoxyribonucleic acid (DNA) relative to the host chromosomal DNA (% R-DNA) was 7% in both exponential and stationary growth phases in Penassay Broth and supplemented M9 minimal medium at 30 C. The chromosomal DNA content per cell varied over a threefold range in the different growth media. In agreement with previous genetic observations, the replication of Rts1 was found to be temperature-sensitive and Rts1 DNA was diluted from the cells during exponential growth at 42 C. ¹⁴N-¹⁵N medium transfer experiments have shown that individual copies of Rts1 are selected at random for replication during the duplication of the multicopy episome pool.

¹ Present address: Department of Microbiology, Institute of Public Health, Shirokanedai, Minato-ku, Tokyo 108, Japan.

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