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Derivation of an F-Merogenote and a 80 High-Frequency Transducing Phage Carrying the Histidine Operon of Salmonella

^a Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218

ABSTRACT

An F' factor, FS400, carrying the his operon, the gnd gene, and the rfb gene cluster of Salmonella typhimurium was isolated. FS400 was introduced into an Escherichia coli strain having a lengthy deletion of the his gene region. From this strain, Hfr derivatives were isolated which had the F' factor integrated in the tonB locus near the attachment site of 80. One of the Hfr strains was lysogenized with a heat-inducible, h mutant of 80, and from this strain a high-frequency transducing phage carrying the his genes and the gnd gene of Salmonella was isolated.

¹ Present address: Microbiology Department, University of Maryland, College Park, Md. 20742.

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