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Characterization of Insoluble Protein Fractions of Mitochondria from Saccharomyces cerevisiae

Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706
Laboratory of Zoology Research, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

Saccharomyces cerevisiae was grown in a chemostat in the presence of excess oxygen. Cells harvested from fully derepressed and strongly repressed steady states show typical promitochondria-like structures under conditions of strong repression. Insoluble membrane proteins were extracted from highly purified mitochondria and submitted to isoelectric focusing in 6% polyacrylamide gels. Some 20 protein bands were obtained from derepressed cells. The pattern was clearly different (quantitatively and possibly qualitatively) from repressed mitochondria. In contrast to ribosomal proteins, insoluble membrane protein fractions were found in the acid section (pH 4 to 6.8) of the ampholyte gels. It can be concluded that glucose repression plays a prominent role in the synthesis of the functional mitochondrial membranes.

² Present address: Rosenstiel Basic Medical Research sische Technische Hochschule, Zurich, Switzerland.

² Present address: Rosenspiel Basic Medical Research Center, Brandeis University, Waltham, Mass. 02154.