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J Bacteriol. 1972 February; 109(2): 949-951
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

p-Toluenethiol as an Initiator of Autolysis in Bakers' Yeast

Wilfred N. Arnold

1 Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66103

ABSTRACT

Cysteine or dithiothreitol enhances the rate of autolysis in toluene-treated yeast. p-Toluenethiol alone is even more effective and is recommended for the isolation of ß-fructofuranosidase. This suggests a more general application of p-toluenethiol in the isolation of enzymes from yeast.

J Bacteriol. 1972 February; 109(2): 949-951
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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