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J Bacteriol. 1972 March; 109(3): 1175-1180
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Acid-Soluble Nucleotides in an Asporogenous Mutant of Bacillus subtilis

^a Department of Biology, McMaster University, Hamilton, Ontario, Canada

ABSTRACT

An asporogenous mutant of Bacillus subtilis Sp^–H12-3, which is considered to have a block at stage 0, showed general growth characteristics similar to those of sporulating cultures. However, a sudden increase in the total amount of acid-soluble nucleotides observed at t₂ in sporulating bacteria was completely absent in this mutant. In sporulating cells, a marked increase in two nucleotides which were identified to be uridine diphosphate (UDP)-galactose and UDP-N-acetylglucosamine was noted, whereas UDP-glucose appeared to be accumulated in the mutant cells at t₂. No unusual nucleotides were found in the strains of B. subtilis examined. The possible role of these UDP derivatives in early stages of sporulation in B. subtilis is discussed.

¹ Present address: Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.