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J Bacteriol. 1972 March; 109(3): 987-992
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Action of Haemophilus Endodeoxyribonuclease on Biologically Active Deoxyribonucleic Acid

¹ Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

ABSTRACT

An enzyme similar to that described by Smith and Wilcox (15) for Haemophilus influenzae which attacks foreign deoxyribonucleic acid (DNA) but not its own has been isolated and purified from H. parainfluenzae. The enzyme degrades foreign DNA to limited sizes and can destroy the transforming activity of H. influenzae and Bacillus subtilis DNA. The enzyme can also destroy the biological activity of H. influenzae phage and prophage DNA. On the other hand, the H. influenzae endodeoxyribonuclease can destroy the transforming activity of H. parainfluenzae DNA but not its own DNA. It also attacks B. subtilis DNA and its transforming activity.

This article has been cited by other articles:

• Nathans, D (1979). Restriction endonucleases, simian virus 40, and the new genetics. Science 206: 903-909  
• Smith, H. (1979). Nucleotide sequence specificity of restriction endonucleases. Science 205: 455-462