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J Bacteriol. 1972 April; 110(1): 110-116
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Identification of Products of the Uridinediphospho-N-acetyl-D-Glucosamine Oxidoreductase System from Citrobacter freundii ATCC 10053

Amiram Daniel1, Rudolf A. Raff2 and Robert W. Wheat

Departments of Biochemistry and Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710

ABSTRACT

Uridinediphospho-N-acetyl glucosamine is converted to one or more uridine-diphospho-4-keto-6-deoxy-2-acetamidohexose derivatives by an enzyme from Citrobacter freundii ATCC 10053. Borohydride reduction of reaction products followed by acid hydrolysis yielded several amino sugars. Two of these were identified as fucosamine and quinovosamine by chromatography and ninhydrin degradation.


FOOTNOTES

1 Present address: Department of Biology, Princeton University, Princeton, N.J. 08540.

2 Department of Biology, University of Indiana, Bloomington, Ind. 47401.


J Bacteriol. 1972 April; 110(1): 110-116
Copyright © 1972 American Society for Microbiology. All Rights Reserved.







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