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J Bacteriol. 1972 April; 110(1): 122-125
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Cell Division of Escherichia coli BUG-6: Effect of Varying the Temperature Used as the Nonpermissive Growth Condition

^a Department of Microbiology, University of British Columbia, Vancouver 8, British Columbia, Canada

ABSTRACT

When Escherichia coli BUG-6 is shifted from 30 C to 36 or 38 C, division does not stop, but the rate of division of the cell population is initially decreased followed by a period of increased rate of division before the rates characteristic of growth at 36 and 38 C are obtained. After a shift from 30 to 40 C, the rate of cell division gradually decreases over a 10-min period and then stops. The inhibition continues for 25 min, and then the cells divide rapidly before the division rate characteristic of 40 C is obtained. If filaments produced by 45 min of growth at 42 C are temporarily replaced at 30 C and then returned to 42 C, division occurs at 42 C. The amount of division is dependent on the length of the period at 30 C and can be decreased by a 3-min pulse of chloramphenicol immediately before the 42 to 30 C shift.

¹ Present address: Department of Microbiology and Medical Technology, University of Arizona, Tucson, Ariz. 85721.

² Present address: Department of Genetics, University of Washington, Seattle, Wash. 98105.