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Temperature-Sensitive Mutants of Escherichia coli K-12 with Low Activity of the Diaminopimelic Acid Adding Enzyme¹

^a Laboratory for Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands

ABSTRACT

Five temperature-sensitive lysis mutants were found to possess very low diaminopimelic acid (Dpm) adding enzyme activity in vitro. Murein synthesis at 42 C was impaired, and uridine-5'-diphosphate-N-acetyl-muramyl-L-Ala- D-Glu (UDP-MurNAc-dipeptide) was accumulated. In the presence of NaCl, the mutants could grow at 42 C. NaCl had no influence on the Dpm adding enzyme activity in vitro. The growth rate of most temperature-resistant revertants was decreased, but their Dpm adding enzyme activity remained very low. Two revertants had a rather normal growth rate. Their Dpm adding enzyme activity was significantly increased, but much lower than in the wild type. The influence of growth rate on the viability of the mutants is discussed.

¹ This paper is taken from a thesis to be submitted by E. J. J. Lugtenberg to the Faculty of Science of the State University in Utrecht in partial fulfillment of the requirements for the Ph.D. degree.

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