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Induction of the ara Operon of Escherichia coli B/r

¹ Department of Botany, University of Michigan, Ann Arbor, Michigan 48104, and Department of Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106

ABSTRACT

The inducer specificity and kinetics of induction of the ara operon were examined in Escherichia coli B/r. A difference in the kinetics of induction was found between our B/r strains and the K-12 strain previously described by Schleif. The roles of active transport and metabolism of inducer, and of cell density, in induction were studied. D-Fucose and ß-methyl-L-arabinoside were competitive inhibitors of induction. No inducer of the wild-type strain other than L-arabinose was found. However, a procedure for selecting mutants with altered inducer affinity or specificity was developed. The properties of one such mutant (inducible by D-fucose) are described.

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