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J Bacteriol. 1972 May; 110(2): 731-738
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Kinetic and Morphological Observations on the Yeast Phase of Histoplasma capsulatum During Protoplast Formation

¹ Center of Microbiology and Cell Biology, Instituto Venezolano de Investigaciones Científicas, Apartado 1827, Caracas, Venezuela, and Department of Microbiology, Harvard School of Public Health, Boston, Massachusetts 02115

ABSTRACT

Protoplast formation by Histoplasma capsulatum yeasts using high concentrations of MgSO₄ occurs either by lysis of the bud or lysis of the entire cell wall. Both mechanisms may also occur simultaneously. Neither the protoplast emerging through a hole in the cell wall nor the freshly released protoplast has a recognizable cell wall or the remnant of such. The protoplast contains all the organelles of the normal cell except for mesosomes. During protoplast formation the nucleus increases in size and produces several nuclear masses by the invaginations of the internal layer of the nuclear membrane. All these nuclear masses are surrounded by the external layer of the nuclear membrane. Several nuclei with a normal nuclear membrane are formed later.