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J Bacteriol. 1972 May; 110(2): 743-746
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Isolation of Morphological Mutants of Agrobacterium tumefaciens

Tamio Fujiwara and Sakuzo Fukui

Institute of Applied Microbiology, University of Tokyo, Tokyo, Japan

ABSTRACT

Morphological mutants were isolated from a wild strain of Agrobacterium tumefaciens at a high frequency by treatment with a nitrosoguanidine. Seventeen of the 20 mutants isolated were temperature-sensitive. At 27 C, the mutant cells were rod-shaped and at 37 C, spherical or branched, whereas the wild-type cells were rod-shaped at both temperatures.

J Bacteriol. 1972 May; 110(2): 743-746
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
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Copyright © 1972 by the American Society for Microbiology. All rights reserved.