This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Baron, L. S. Articles by Gemski, P. Search for Related Content PubMed PubMed Citation Articles by Baron, L. S. Articles by Gemski, P., Jr.

Lytic Replication of Coliphage Lambda in Salmonella typhosa Hybrids

¹ Department of Bacterial Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012

ABSTRACT

Hybrids between Escherichia coli K-12 and Salmonella typhosa which conserved a continuous K-12 chromosomal diploid segment extending from pro through ara to the strA locus were sensitive to plaque formation by wild-type . These partially diploid S. typhosa hybrids could be lysogenized with and subsequently induced to produce infectious phage particles. When the K-12 genes were segregated from a lysogenic S. typhosa hybrid, phage-productive ability was no longer detectable due to loss of a genetic region necessary for vegetative replication of . However, prophage was shown to persist in a quiescent state in the S. typhosa hybrid segregant with phage-productive ability being reactivated after replacement of the essential K-12 replication region. Low-frequency transduction and high-frequency transduction lysates containing the gal⁺ genes of S. typhosa were prepared by induction of -lysogenic S. typhosa hybrids indicating that the att site is chromosomally located in S. typhosa in close proximity to the gal locus as in E. coli K-12. After propagation in S. typhosa hybrids, was subject to restriction by E. coli K-12 recipients, thus establishing that S. typhosa does not perform the K-12 modification of deoxyribonucleic acid. Hybrids of S. typhosa, however, did not restrict grown previously on E. coli K-12. The K-12 genetic region required for phage production in S. typhosa was located within min 66 to min 72 on the genetic map of the E. coli chromosome. Transfer of an F-merogenote encompassing the 66 to 72 min E. coli chromosomal region to -insensitive S. typhosa hybrids enabled them to replicate wild-type . The -insensitive S. typhosa hybrid, WR4255, which blocks replication, can be mutagenized to yield mutant strains sensitive to vir and imm434. These WR4255 mutants remained insensitive to plaque formation by wild-type .

This article has been cited by other articles:

• Zahrt, T. C., Maloy, S. (1997). Barriers to recombination between closely related bacteria: MutS and RecBCD inhibit recombination between Salmonella typhimurium and Salmonella typhi. Proc. Natl. Acad. Sci. USA 94: 9786-9791 [Abstract] [Full Text]