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J Bacteriol. 1972 June; 110(3): 1208-1210
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Efficiency of Thymidine Incorporation in Escherichia coli B/r as a Function of Growth Rate

H. E. Kubitschek, D. Valdez and M. L. Freedman1

a Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60440

ABSTRACT

Short-term labeling with radioactive thymidine led to inaccurate estimates of deoxyribonucleic acid synthesis at some growth rates in steady-state cultures of Escherichia coli B/r. Estimates were corrected in chemostat cultures by adding adenosine, a known inhibitor of thymidine phosphorylase.

FOOTNOTES

1 Present address: Department of Oral Radiology, McCook Hospital, Hartford, Conn. 06112.

J Bacteriol. 1972 June; 110(3): 1208-1210
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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