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Conjugational Complementation Analysis of Transfer-Deficient Mutants of Flac in Escherichia coli

^a MRC Molecular Genetics Unit, Department of Molecular Biology, University of Edinburgh, Edinburgh, Scotland, and Department of Molecular Biology, University of California, Berkeley, California 94720

ABSTRACT

A series of 102 transfer-deficient (tra^–) mutants of Flac (84 of which have been previously described) were classified as carrying frameshift, amber, ochre, UGA, and nonsuppressible mutations. Techniques were evolved for using cultures of F prime strains as efficient recipients in matings, for measuring the number of (F'/F') heterozygote cells in transient populations, and for measuring complementation between different Flac tra^– mutants. These techniques were used to define nine tra cistrons and to assign most of the tra^– mutations to one or other of these.

¹ Present address: Max-Planck Institut für Biologie, 74 Tübingen Corrensstrasse 38, West Germany.

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