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J Bacteriol. 1972 June; 110(3): 935-944
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of a Phosphonomycin-Resistant Mutant of Escherichia coli K-12

Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06032

ABSTRACT

A mutant was isolated from Escherichia coli K-12 which showed increased resistance towards phosphonomycin, a new bactericidal antibiotic recently isolated from strains of Streptomyces. Evidence is presented which suggests that this mutant is resistant to lysis by phosphonomycin because of a lower affinity of phosphoenolpyruvate: uridine diphospho-N-acetylglucosamine enolpyruvyl transferase for this antibiotic. This mutant was also found to be temperature-sensitive in growth. At 42 C mutant cells grew poorly, and the rate of incorporation of ³H-diaminopimelic acid into trichloroacetic acid-insoluble material was also greatly reduced. Genetic studies indicate that the increased resistance toward phosphonomycin and temperature sensitivity in growth of this mutant are probably the consequences of a single mutation.

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