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J Bacteriol. 1972 July; 111(1): 242-247
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Axenic Culture of Myxamoebae of the Myxomycete Physarum polycephalum

E. M. Goodman

1 University of Wisconsin-Parkside, Kenosha, Wisconsin 53140

ABSTRACT

Myxamoebae of the acellular slime mold Physarum polycephalum have been cultured axenically in a soluble medium. The growth medium contains bovine serum albumin, embryo extracts, liver infusion broth, peptone, and glucose. Cell densities ranging from 3 x 106 to 5 x 106 cells/ml have been obtained with this medium. To date, myxamoebae have been serially transferred more than 100 times without deleterious effect.

J Bacteriol. 1972 July; 111(1): 242-247
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
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Copyright © 1972 by the American Society for Microbiology. All rights reserved.