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J Bacteriol. 1972 July; 111(1): 248-253
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Ultrastructural Analysis of Sporulation in a Conditional Serine Protease Mutant of Bacillus subtilis¹

^a Department of Biochemistry and Biophysics, University of California, Davis, California 95616

ABSTRACT

The morphological characteristics of wild-type Bacillus subtilis and a temperature-sensitive serine protease derivative have been observed during vegetative and sporulation time periods. At 30 C wild-type and mutant cells grow and sporulate identically. At 47.5 C wild-type and mutant cells grow identically, but the mutant cells are blocked at stage 0 or I in the sporulation sequence. Wild-type cells sporulate normally at 47.5 C.

¹ A preliminary report covering this work was presented at the 71st Annual Meeting of the American Society for Microbiology, Minneapolis, Minnesota, 2–7 May 1971.