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Mapping of rod Mutants of Bacillus subtilis

National Institute for Medical Research, Mill Hill, London, NW7, England

ABSTRACT

Nine class A salt-dependent rod mutants were mapped on the Bacillus subtilis genome by PBS1-mediated transduction. They are distributed into two small linkage groups designated rod B and rod C; mutations in rod B are over 80% cotransducible with pheA and different mutations in rod C are 12 to 21% cotransducible with hisA. It is established that neither rod B nor rod C is linked by transformation to the other identified rod mutations present in 168-ts-200B and 8332 glu^–. It is hypothesized that salt-dependent mutations are due to enzyme alterations which are corrected by high salt concentrations.

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