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J Bacteriol. 1972 August; 111(2): 454-458
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Evidence for a Phosphoenolpyruvate-Dependent Sugar Phosphotransferase in Mycoplasma Strain Y

^a CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria 3052, Australia

ABSTRACT

The uptake of ¹⁴C--methyl-D-glucoside (MG) by washed cells of Mycoplasma strain Y was found to be dependent on the supply of metabolic energy. Glycerol or D-mannose, but not L-lactate, would serve as an energy source. Uptake was inhibited by fluoride, iodoacetate, and arsenate, but not by 2,4-dinitrophenol. D-Glucose was inhibitory, presumably by competing for the transport system. The initial product of accumulation had the properties of a phosphate ester of MG. The proportion of free MG in the cells increased with time, until a steady state was reached in which uptake was balanced by the efflux of free MG from the cells. Broken-cell preparations catalyzed a phosphoenolpyruvate-dependent phosphorylation of MG and of D-glucose.

¹ Present address: Microbiology Section, Cornell University, Ithaca, N.Y. 14850.