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J Bacteriol. 1972 August; 111(2): 465-473
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Inducible Uptake System for ß-Carboxy-cis, cis-muconate in a Permeability Mutant of Pseudomonas putida

Department of Biology, Yale University, New Haven, Connecticut 06520

ABSTRACT

A procedure for the large-scale enzymatic synthesis of ß-carboxymuconate is described. When used as a growth substrate, ß-carboxymuconate selected for mutant strains of Pseudomonas putida that were permeable to polycarboxylic acid intermediates of the ß-ketoadipate pathway. One mutant organism, strain PRS2110, was investigated in detail. It differed from the parental strain in that it possessed a ß-carboxymuconate uptake system that was formed when the compound was supplied exogenously to the cells. The uptake system was not induced by ß-carboxymuconate supplied endogenously during growth with p-hydroxybenzoate. These observations suggested that ß-carboxymuconate was contained within a physical compartment of enzymes during growth with p-hydroxybenzoate. Support for this hypothesis came from the demonstration that enzymes of the ß-ketoadipate pathway were held together by weak chemical interactions during the chromatography of crude extracts of benzoategrown P. putida on diethylaminoethyl-cellulose columns.

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