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Fate of Transforming Deoxyribonucleic Acid After Uptake by Competent Bacillus subtilis: Size and Distribution of the Integrated Donor Segments

¹ Department of Microbiology, The Public Health Research Institute of the City of New York, Inc., New York, New York 10016

ABSTRACT

A series of sheared donor-recipient complex samples were prepared from a competent Bacillus subtilis culture which had been transformed by using ³H, ²H-labeled deoxyribonucleic acid. Measurement of the molecular weights and buoyant densities of these samples led to the following conclusions. (i) The average size of the single-stranded donor segment which is integrated is 2.8 x 10⁶. (ii) These donor segments are not randomly distributed in the recipient genome, but are clustered in groups, each group containing several discrete donor segments.

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