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J Bacteriol. 1972 September; 111(3): 696-704
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Isolation of Minicircular Deoxyribonucleic Acids from Wild Strains of Escherichia coli and their Relationship to other Bacterial Plasmids

Abteilung für Mikrobiologie und Molekularbiologie der Universität Hohenheim, Stuttgart-Hohenheim, Germany

ABSTRACT

Supercoiled minicircular deoxyribonucleic acid (DNA) molecules with molecular weights of 1.8 x 10⁶ and 2.3 x 10⁶ have been isolated from two wild strains of Escherichia coli. DNA-DNA hybridization experiments indicate that these DNA molecules share extended homologies with the minicircular DNA of E. coli 15. The DNA of the colicinogenic factor E1 (ColE1) also hybridizes to a large extent with minicircular DNA of E. coli 15. In contrast, no hybridization could be detected with various large extrachromosomal DNA elements such as the colicinogenic factor V (ColV), the beta-hemolytic factor (Hly), or the P1-like DNA of E. coli 15. Two different insertion DNA species of E. coli integrated into dg-DNA (dg UP_in 128, dg UP_in 308) do not show any annealing with minicircular DNA of E. coli 15.