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J Bacteriol. 1972 September; 111(3): 739-744
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Regulation of Histidyl-Transfer Ribonucleic Acid Synthetase Formation in a Histidyl-Transfer Ribonucleic Acid Synthetase Mutant of Salmonella typhimurium

Department of Biological Sciences, Purdue University, Lafayette, Indiana 47907

ABSTRACT

Control of formation of the histidyl-transfer ribonucleic acid (tRNA) synthetase with an increased K_m for histidine was studied in a hisS mutant of Salmonella typhimurium. Histidine restriction of both the hisS and hisS⁺ strains resulted in a derepression of synthesis of histidyl-tRNA synthetase. When grown in a concentration less than the K_m (100 µg/ml) of L-histidine, the hisS mutant maintained a higher level of histidyl-tRNA synthetase than the hisS⁺ strain. Addition of excess amounts of L-histidine to the growth medium of the hisS mutant culture grown with 100 µg of L-histidine per ml resulted in a repression of histidyl-tRNA synthetase formation to equal that of the hisS⁺ strain grown in 100 µg of L-histidine per ml. These data confirm previous findings that histidine tRNA is involved in the repression of synthesis of histidyl-tRNA synthetase.