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J Bacteriol. 1972 September; 111(3): 791-796
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Antibody-Mediated Modification of the Binding Properties of a Protein Related to Galactose Transport

¹ Division of Biological and Medical Sciences, Brown University, Providence, Rhode Island 02912

ABSTRACT

A galactose-binding protein related to the mglP transport system of Escherichia coli increases its affinity and binding capacity for the substrate when exposed to both D-galactose and specific antibodies. For this increase to occur, the binding protein has to be in contact with D-galactose for at least 2 min prior to the addition of the antibodies. This reaction was used to show that other substrates of the mglP transport system compete with galactose for a site(s) of the binding protein and that the degree of competition is comparable to that observed in vivo. A model for substrate translocation is presented postulating a cellular component that can induce conformational changes in the galactose-binding protein similar to those caused by antibodies.

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