This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Morrison, D. A. Articles by Guild, W. R. Search for Related Content PubMed PubMed Citation Articles by Morrison, D. A. Articles by Guild, W. R.

Activity of Deoxyribonucleic Acid Fragments of Defined Size in Bacillus subtilis Transformation

¹ Department of Biochemistry, Duke University, Durham, North Carolina 27710

ABSTRACT

The transforming activity of Bacillus subtilis deoxyribonucleic acid (DNA) that had been sheared and purified with respect to size by sucrose gradient sedimentation is given as a function of the DNA molecular weight. It is shown (i) that fragments of median molecular weight 1.2 x 10⁶ have finite activity (10^–4), (ii) that the shape of the activity-versus-molecular weight function is qualitatively similar to that observed previously for Diplococcus pneumoniae, and (iii) that this shape precludes interpretation in terms of critical size models.

This article has been cited by other articles:

• Levine, S. M., Lin, E. A., Emara, W., Kang, J., DiBenedetto, M., Ando, T., Falush, D., Blaser, M. J. (2007). Plastic cells and populations: DNA substrate characteristics in Helicobacter pylori transformation define a flexible but conservative system for genomic variation. FASEB J. 21: 3458-3467 [Abstract] [Full Text]