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J Bacteriol. 1972 October; 112(1): 513-518
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Metabolism of Propane, n-Propylamine, and Propionate by Hydrocarbon-Utilizing Bacteria¹

^a Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27607

ABSTRACT

Studies were conducted on the oxidation and assimilation of various three-carbon compounds by a gram-positive rod isolated from soil and designated strain R-22. This organism can utilize propane, propionate, or n-propylamine as sole source of carbon and energy. Respiration rates, enzyme assays, and ¹⁴CO₂ incorporation experiments suggest that propane is metabolized via methyl ketone formation; propionate and n-propylamine are metabolized via the methylmalonyl-succinate pathway. Isocitrate lyase activity was found in cells grown on acetate and was not present in cells grown on propionate or n-propylamine. ¹⁴CO₂ was incorporated into pyruvate when propionate and n-propylamine were oxidized in the presence of NaAsO₂, but insignificant radioactivity was found in pyruvate produced during the oxidation of propane and acetone. The n-propylamine dissimilatory mechanism was inducible in strain R-22, and amine dehydrogenase activity was detected in cells grown on n-propylamine. Radiorespirometer and ¹⁴CO₂ incorporation studies with several propane-utilizing organisms indicate that the methylmalonyl-succinate pathway is the predominant one for the metabolism of propionate.

¹ Paper no. 3529 of the Journal Series of the North Carolina State University Agricultural Experiment Station, Raleigh, N.C. 27607.

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