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J Bacteriol. 1972 October; 112(1): 527-531
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Isolation and Properties of a Fluorescent Compound, Factor₄₂₀, from Methanobacterium Strain M.o.H

¹ Department of Microbiology, University of Illinois, Urbana, Illinois 61801

ABSTRACT

A new fluorescent compound, factor₄₂₀ (F₄₂₀), which is involved in the hydrogen metabolism of hydrogen-grown Methanobacterium strain M.o.H. has been isolated and purified. Acid hydrolysis of this compound with 6 M HCl for 24 hr releases a ninhydrin-positive compound (glutamic acid), an acid-stable chromophore, phosphate, and an ether-soluble phenolic component. Factor₄₂₀ may be reduced by either sodium dithionite or sodium borohydride at pH 7.3 with concomitant loss of its fluorescence and its major absorption peak at 420 nm. Crude cell-free extracts of strain M.o.H. reduce F₄₂₀ only under a hydrogen atmosphere. F₄₂₀ is photolabile aerobically in neutral and basic solutions, whereas the acid-stable chromophore is not photolabile under these conditions. An approximate molecular weight of 630 ± 8% for F₄₂₀ was determined by Sephadex G-25 chromatography. At the present time, F₄₂₀ is proposed as a trivial name for the unknown fluorescent compound because of its strong absorption maximum of 420 nm at pH 7.

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