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J Bacteriol. 1972 October; 112(1): 649-652
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Toxicity of Irradiated Medium for Repair-Deficient Strains of Escherichia coli

Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60439

ABSTRACT

Nutrient agar medium is made highly toxic to certain repair-deficient strains of Escherichia coli by exposure of the uninoculated plates to radiation from cool-white fluorescent lamps or black-light fluorescent lamps. This sensitivity is associated with the genetic deficiencies, fil, phr, and recA. Repair-sufficient and uvr strains are only slightly affected by the irradiated media. The poor growth and reduced plating efficiency frequently associated with BPhr and recA strains are very likely caused by inadvertent exposure of the medium to fluorescent light.