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Temperature-Sensitive Osmotic Remedial Mutants of Escherichia coli

¹ CSIRO Division of Food Research, Meat Research Laboratory, Cannon Hill, Queensland, Australia 4170

ABSTRACT

A collection of temperature-sensitive mutants of Escherichia coli K-12 was examined for ability to grow at the restrictive temperature when the osmotic pressure of the medium was increased. Five of the fourteen mutants were found to be osmotic remedial. Four strains containing temperature-sensitive, osmotic-remedial mutations affecting aminoacyl-transfer ribonucleic acid synthetases were found to have altered permeability characteristics which may be attributable to changes in the lipopolysaccharide layer of the cell envelope at restrictive temperatures.

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