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J Bacteriol. 1972 November; 112(2): 761-772
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Genetic Studies on Plasmid-Linked Cadmium Resistance in Staphylococcus aureus

^a The Public Health Research Institute of the City of New York, Inc., New York, New York 10016

ABSTRACT

The genetic basis of cadmium resistance conferred by three penicillinase plasmids, PI₅₂₄, PI₂₅₈, and PII₁₄₇, of Staphylococcus aureus was examined by mutation, recombination, and deletion analysis. Three separate loci were identified: cadA, responsible for high-level resistance; cadB, giving a low-level resistance, nonadditive to cadA; and mad, a locus marginally decreasing the cadmium resistance of plasmid-positive staphylococci. The loci cadA and mad were present on all three plasmids, but cadB was only found on PII₁₄₇. Spontaneous deletions of mad involved up to three-fourths of the plasmid genome, which allowed derivation of a partial deletion map of PII₁₄₇, a plasmid with a contour length of 10.9 µm, corresponding to a molecular weight of 20.4 x 10⁶.

¹ Present address: 80 Pentland Terrace, Edinburgh 10, Scotland.

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