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J Bacteriol. 1972 November; 112(2): 886-893
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Dominant Mutations (lex) in Escherichia coli K-12 Which Affect Radiation Sensitivity and Frequency of Ultraviolet Light-Induced Mutations

David W. Mount1, K. Brooks Low2 and Susan J. Edmiston3

Department of Microbiology, College of Medicine, University of Arizona, Tucson, Arizona 85724
Department of Radiology, Yale University School of Medicine, New Haven, Connecticut 06510
Department of Microbiology, Yale University School of Medicine, New Haven, Connecticut 06510

ABSTRACT

Three mutations, denoted lex-1, -2 and -3, which increase the sensitivity of Escherichia coli K-12 to ultraviolet light (UV) and ionizing radiation, have been found by three-factor transduction crosses to be closely linked to uvrA on the E. coli K-12 linkage map. Strains bearing these mutations do not appear to be defective in genetic recombination although in some conjugational crosses they may fail to produce a normal yield of genetic recombinants depending upon the time of mating and the marker selected. The mutagenic activity of UV is decreased in the mutant strains. After irradiation with UV, cultures of the strains degrade their deoxyribonucleic acid at a high rate, similar to recA– mutant strains. Stable lex+/lec– heterozygotes are found to have the mutant radiation-sensitive phenotype of haploid lex– strains.

J Bacteriol. 1972 November; 112(2): 886-893
Copyright © 1972 American Society for Microbiology. All Rights Reserved.



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