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Cell Wall Peptidoglycan Mutants of Escherichia coli K-12: Existence of Two Clusters of Genes, mra and mrb, for Cell Wall Peptidoglycan Biosynthesis

^a Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Tokyo, Japan, and Department of Biology, Faculty of Science, Osaka University, Osaka, Japan

ABSTRACT

Temperature-sensitive mutants of Escherichia coli K-12 which cannot form cell wall peptidoglycan at 42 C were isolated. The thermosensitive steps were characterized biochemically, and the genes coding the enzymes of peptidoglycan synthesis were mapped. These genes were in two clusters: one group, located at about 1.5 min between leu and azi, was designated as mra (murein a); the second group, located at about 77.5 min close to argH and metB, was designated as mrb (murein b, with the order metB-argH-mrb). No simple relations were found between the gene location and the order or localization of enzymes involved in the sequence of reactions of cell wall peptidoglycan biosynthesis.

¹ Present address: Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo, Japan.

² On leave of absence at the National Institute of Arthritis and Metabolic Diseases, Bethesda, Md.

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