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J Bacteriol. 1972 December; 112(3): 1270-1274
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Template Specificity of Ribonucleic Acid Polymerase in Asporogenous Mutants of Bacillus subtilis

^a Institut de Microbiologie, Université Paris-Sud, Centre D'Orsay (91), France

ABSTRACT

Asporogenous mutants of Bacillus subtilis were examined for the change in template specificity of ribonucleic acid (RNA) polymerase characteristic of wild-type cells undergoing sporulation. Mutants blocked at stages II, III, and IV showed a changed specificity of the enzyme after the end of growth and were in this respect indistinguishable from the wild type. The RNA polymerase of eight stage-zero mutants (out of nine tested) which possess mutations that map at six distinct loci retained the template specificity of vegetative cells.

¹ Present address: Department of Molecular Biology and Microbiology. Tufts University School of Medicine, Boston, Mass. 02111.