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Isolation of Covalently Closed Circular Deoxyribonucleic Acid from Bacteria Which Produce Exocellular Nuclease

^a Lehrstuhl für Biologie der Mikroorganismen, Ruhr Universität Bochum, 4630 Bochum, Germany

ABSTRACT

Reproducible yields of covalently closed circular (plasmid) deoxyribonucleic acid were obtained from mutants defective for extracellular nuclease but not from the corresponding wild-type strain of Serratia marcescens

¹ Present address: Department of Molecular Biophysics and Biochemistry, Yale University, 1937 Yale Station, New Haven, Conn. 06520.

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