This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hadden, C. T. Articles by Billen, D. Search for Related Content PubMed PubMed Citation Articles by Hadden, C. T. Articles by Billen, D.

Genetic Analysis of Repair of Ultraviolet Damage by Competent and Noncompetent Cells of Bacillus subtilis

¹ Division of Radiation Biology, Department of Radiology, College of Medicine, University of Florida, Gainesville, Florida 32601
² Department of Immunology and Medical Microbiology, College of Medicine, University of Florida, Gainesville, Florida 32601

ABSTRACT

The repair of ultraviolet (UV) damage in Bacillus subtilis W23T^– has been studied by transformation with deoxyribonucleic acid (DNA) extracted from irradiated cells before and after repair. The extent of repair of genetic markers by donor cells after low or moderate doses of UV was found to be related only to the initial degree of inactivation. After a very high dose, further inactivation occurred, also in proportion to initial damage. In addition, the competent recipient cells were shown to repair approximately 75% of the damage in transforming DNA. The sensitivities of markers irradiated either in vivo or in vitro appeared to be related to map position, the more proximal markers showing a greater resistance to UV inactivation.