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Control of Pantothenate Accumulation in Agrobacterium tumefaciens

^a Northern Regional Research Laboratory, Northern Marketing and Nutrition Research Division, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604

ABSTRACT

Two pantothenate-requiring mutants of Agrobacterium tumefaciens have been isolated. One of them (strain WMP-1) is unusual in that growth levels equivalent to the parent strain are achieved only when the medium is additionally supplemented with aspartate or another compound related to the tricarboxylic acid cycle. Extracts of cells grown on limiting aspartate were found to contain four times more ¹⁴C-pantothenate than those grown at optimal aspartate concentrations. This difference was found in both the perchloric acid-soluble and -insoluble fractions, presumably the coenzyme A pool and acyl carrier protein, respectively. These findings are discussed in terms of membrane integrity and the control of fatty acid biosynthesis.

¹ Postdoctoral Research Associate.

² Present address: Department of Health Sciences, Grand Valley State College, Allendale, Mich. 49401.