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Temperature-Sensitive Divisionless Mutant of Bacillus subtilis Defective in the Initiation of Septation

^a Department of Biology, Georgetown University, Washington D.C. 20007

ABSTRACT

A temperature-sensitive divisionless mutant of Bacillus subtilis 168, tms-12, is shown to be defective in an early step in septum formation at the restrictive temperature. The nature of this defect has been studied by comparing the growth and composition of mutant and wild-type (tms-12⁺) cells at the restrictive (48 C) and permissive (34 C) temperatures. At 48 C, tms-12 cells grow as nonseptate, multinucleate filaments. Filamentation does not appear to be a result of alterations in properties of the cell wall, since the ratio of mucopeptide to teichoic acid, the autolytic activity, and the ability of the walls to protect cells against osmotic shock are comparable in tms-12 filaments and tms-12⁺ bacilli grown at 48 C. Synthesis of deoxyribonucleic acid and the segregation of nucleoids also proceed normally during filamentation. The synthesis of membrane, however, is delayed during filamentation of tms-12. No gross alterations were observed in the protein or lipid composition of membranes isolated from mutant filaments. Septum formation resumes when filaments are returned to 34 C and appears to be associated with an increased synthesis of membrane. The occurrence of septa was monitored both by microscopic observation of cross walls and by assays of the number of viable protoplasts released from bacillary filaments upon removal of the cell wall. Septation recovery can be blocked by inhibitors of ribonucleic acid and protein synthesis added during, but not after, the first 7 min of recovery at 34 C. By contrast, inhibition of deoxyribonucleic synthesis does not block recovery.

¹ Present address: Laboratory of Biochemical Genetics, Heart and Lung Institute, National Institutes of Health, Bethesda, Md. 20014.

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