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Close Linkage of the Genes serC (for Phosphohydroxy Pyruvate Transaminase) and serS (for Seryl-Transfer Ribonucleic Acid Synthetase) in Escherichia coli K-12

^a Department of Molecular Biophysics, Yale University, New Haven, Connecticut 06510
Department of Biochemistry, Yale University, New Haven, Connecticut 06510
^c Department of Radiology, Yale University, New Haven, Connecticut 06510

ABSTRACT

Escherichia coli strain K28, isolated after nitrosoguanidine mutagenesis, was found to be auxotrophic for serine. It was also temperature sensitive for growth as a result of producing an altered seryl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.11, L-serine: tRNA ligase [AMP]). The auxotrophy was caused by a mutation in the structural gene for phosphohydroxy-pyruvate transaminase (serC), which was distinct from, but closely linked to, the structural gene for seryl-tRNA synthetase (serS). We conclude that the relevant genes are in the order gal-serS-serC-aroA.

¹ Present address: Department of Biochemistry, University of the West Indies, Kingston, Jamaica.

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