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Relationship Between Methionyl Transfer Ribonucleic Acid Cellular Content and Synthesis of Methionine Enzymes in Saccharomyces cerevisiae

¹ Laboratoire d'Enzymologie, C.N.R.S., 91190 Gif-sur-Yvette, France

ABSTRACT

Derepression of some methionine biosynthetic enzymes (methionine group I enzymes) obtained in methionine limitation has been found to be accompanied by a significant lack of in vivo charging of bulk methionine transfer ribonucleic acid (tRNA^Met) and in addition by a decreased rate of synthesis of all tRNAs. Under the same conditions, methionyl-tRNA synthetase (MTS) was derepressed rather than repressed. These results are in agreement with those previously published based on studies of a mutant with an impaired MTS (5) and reinforce the idea that the rate of synthesis of methionine group I enzymes can be related to the total content of methionyl (Met)-tRNA ^Met per cell. They also render unlikely that MTS could be a constituent of the regulatory signal.