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Macromolecular Synthesis During the Germination of Saccharomyces cerevisiae Spores

^a Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706,
Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02154

ABSTRACT

After the dormancy of Saccharomyces cerevisiae ascospores had been broken, the synthesis of proteins was observed first, followed rapidly by synthesis of ribonucleic acid (RNA) and much later by deoxyribonucleic acid (DNA) synthesis. Phosphoglucomutase activity increased in a periodic (step) fashion, whereas the activity of five other enzymes increased linearly during germination and outgrowth. The rate of synthesis of these enzymes was highest at about the period of DNA replication. The amino acid pools of dormant spores contained high levels of proline, glutamic acid, and histidine. At 2 h after onset of germination, the pools of phenylalanine and methionine had disappeared and the other components had decreased significantly. By 3.5 h, with the exception of proline and cystine, most amino acid pool components had significantly increased.

¹ Present address: Research and Development Laboratories, Canada Packers Limited, Toronto 9, Ontario, Canada.

This article has been cited by other articles:

• Brengues, M., Pintard, L., Lapeyre, B. (2002). mRNA Decay Is Rapidly Induced after Spore Germination of Saccharomyces cerevisiae. J. Biol. Chem. 277: 40505-40512 [Abstract] [Full Text]