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J Bacteriol. 1973 March; 113(3): 1345-1347
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Proton Magnetic Resonance Spectroscopy of Klebsiella Capsular Polysaccharides

Department of Chemistry, University of British Columbia, Vancouver 8, B.C., Canada

ABSTRACT

The presence of acetate and pyruvate groups in Klebsiella capsular polysaccharides may be demonstrated and estimated quantitatively by running the proton magnetic resonance spectrum of the polysaccharide (as sodium salt) in deuterium oxide at 95 C. Such spectra also permit an assessment to be made of the number of - and ß-linkages in the repeat unit of the polysaccharide structure.

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