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Isolation and Characterization of Thermosensitive Escherichia coli Mutants Defective in Deoxyribonucleic Acid Replication

¹ Department of Biological Sciences, Columbia University, New York, New York 10027; Max-Planck-Institut für Virusforschung, 74 Tübingen, Germany; and Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, 74 Tübingen, Germany

ABSTRACT

Thermosensitive deoxyribonucleic acid replication-defective mutants have been isolated by using an autoradiographic selection method. The mutants have been analyzed genetically and biochemically. Some of the mutants show thermosensitivity of in vitro deoxyribonucleic acid replication. These can be classified into three groups according to their behavior in in vitro complementation assays. This classification is congruent with that obtained by genetic mapping by using cotransduction frequencies with selected markers in P1 transduction analysis.

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