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J Bacteriol. 1973 April; 114(1): 1-6
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Nuclear Deoxyribonucleic Acid-Dependent Ribonucleic Acid Polymerases from Saccharomyces cerevisiae

¹ Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02154

ABSTRACT

Two deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerases (I, II) have been solubilized from isolated Saccharomyces cerevisiae nuclei. The enzymes can be separated by chromatography on O-diethylaminoethyl Sephadex. Both enzymes are active with high-molecular-weight nuclear yeast DNA, although RNA polymerase I has a higher affinity for polydeoxy-adenylic-thymidylic acid and RNA polymerase II for denatured DNA. RNA polymerase I is active only with manganese. -Amanitin inhibits only the activity of RNA polymerase II.