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J Bacteriol. 1973 May; 114(2): 798-803
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Mutant of Bacillus subtilis with a Temperature-Sensitive Autolytic Amidase

David P. Fan and Mary M. Beckman

1 Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101

ABSTRACT

A procedure incorporating a petri plate assay for autolysins has been developed for the systematic isolation of mutants with a temperature-sensitive autolytic amidase. We suggest maa as the name for the gene for this enzyme. One such mutant has been characterized as having a heat-sensitive amidase in a variety of studies with both crude autolysin preparations and purified amidase solutions.

J Bacteriol. 1973 May; 114(2): 798-803
Copyright © 1973 American Society for Microbiology. All Rights Reserved.





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Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
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Copyright © 1973 by the American Society for Microbiology. All rights reserved.