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J Bacteriol. 1973 June; 114(3): 1271-1280
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Ultrastructural Study of Poly-ß-Hydroxybutyrate Granules from Bacillus cereus

Department of Biology, University of York, Heslington, York, England

ABSTRACT

The freeze-etching technique was used to examine the effects of fracturing and etching on the appearance of poly-ß-hydroxybutyrate granules from Bacillus cereus. These granules were examined in extracts isolated by hypochlorite or by sonic treatment, and also in fixed and unfixed intact cells; in the latter case they were compared with granules in thin sections of intact cells. After freeze-fracturing, the diameter of the granules in intact cells was between 240 and 720 nm. The granules consisted of a central core, of diameter between 140 and 370 nm, which occupied less than 50% of the volume of the granule and which was either stretched or removed on fracturing; the core was surrounded by an outer coat which may be bounded by a membrane.

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