This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dunn, N. W. Articles by Gunsalus, I. C. Search for Related Content PubMed PubMed Citation Articles by Dunn, N. W. Articles by Gunsalus, I. C.

Transmissible Plasmid Coding Early Enzymes of Naphthalene Oxidation in Pseudomonas putida

¹ School of Chemical Sciences, University of Illinois, Urbana, Illinois 61801

ABSTRACT

The capacity of Pseudomonas putida PpG7 (ATCC 17,485) to grow on naphthalene, phenotype Nah⁺, is lost spontaneously, and the frequency is increased by treatment with mitomycin C. The Nah⁺ growth character can be transferred to cured or heterologous fluorescent pseudomonads lacking this capacity by conjugation, or between phage pf16-sensitive strains by transduction. After mutagenesis, strains can be selected with increased donor capacity in conjugation. Clones which use naphthalene grow on salicylate and carry catechol 2,3-oxygenase, the initial enzyme of the aromatic -keto acid pathway, whereas cured strains grow neither on salicylate nor naphthalene and lack catechol 2,3-oxygenase, but retain catechol 1,2-oxygenase and the aromatic ß-keto adipate pathway enzymes.

This article has been cited by other articles:

• Gomes, N. C. M., Borges, L. R., Paranhos, R., Pinto, F. N., Krogerrecklenfort, E., Mendonca-Hagler, L. C. S., Smalla, K. (2007). Diversity of ndo Genes in Mangrove Sediments Exposed to Different Sources of Polycyclic Aromatic Hydrocarbon Pollution. Appl. Environ. Microbiol. 73: 7392-7399 [Abstract] [Full Text]  
• Sota, M., Yano, H., Ono, A., Miyazaki, R., Ishii, H., Genka, H., Top, E. M., Tsuda, M. (2006). Genomic and Functional Analysis of the IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Its Transposon Tn4655 Suggests Catabolic Gene Spread by a Tyrosine Recombinase. J. Bacteriol. 188: 4057-4067 [Abstract] [Full Text]  
• Shintani, M., Yano, H., Habe, H., Omori, T., Yamane, H., Tsuda, M., Nojiri, H. (2006). Characterization of the Replication, Maintenance, and Transfer Features of the IncP-7 Plasmid pCAR1, Which Carries Genes Involved in Carbazole and Dioxin Degradation.. Appl. Environ. Microbiol. 72: 3206-3216 [Abstract] [Full Text]  
• Jeon, C. O., Park, M., Ro, H.-S., Park, W., Madsen, E. L. (2006). The Naphthalene Catabolic (nag) Genes of Polaromonas naphthalenivorans CJ2: Evolutionary Implications for Two Gene Clusters and Novel Regulatory Control. Appl. Environ. Microbiol. 72: 1086-1095 [Abstract] [Full Text]  
• Kulakov, L. A., Chen, S., Allen, C. C. R., Larkin, M. J. (2005). Web-Type Evolution of Rhodococcus Gene Clusters Associated with Utilization of Naphthalene. Appl. Environ. Microbiol. 71: 1754-1764 [Abstract] [Full Text]  
• Hearn, E. M., Dennis, J. J., Gray, M. R., Foght, J. M. (2003). Identification and Characterization of the emhABC Efflux System for Polycyclic Aromatic Hydrocarbons in Pseudomonas fluorescens cLP6a. J. Bacteriol. 185: 6233-6240 [Abstract] [Full Text]  
• Jeong, J. J., Kim, J. H., Kim, C.-K., Hwang, I., Lee, K. (2003). 3- and 4-alkylphenol degradation pathway in Pseudomonas sp. strain KL28: genetic organization of the lap gene cluster and substrate specificities of phenol hydroxylase and catechol 2,3-dioxygenase. Microbiology 149: 3265-3277 [Abstract] [Full Text]  
• Lessner, D. J., Parales, R. E., Narayan, S., Gibson, D. T. (2003). Expression of the Nitroarene Dioxygenase Genes in Comamonas sp. Strain JS765 and Acidovorax sp. Strain JS42 Is Induced by Multiple Aromatic Compounds. J. Bacteriol. 185: 3895-3904 [Abstract] [Full Text]  
• Park, W., Jeon, C. O., Hohnstock-Ashe, A. M., Winans, S. C., Zylstra, G. J., Madsen, E. L. (2003). Identification and Characterization of the Conjugal Transfer Region of the pCg1 plasmid from Naphthalene-Degrading Pseudomonas putida Cg1. Appl. Environ. Microbiol. 69: 3263-3271 [Abstract] [Full Text]  
• Ji, P., Wilson, M. (2003). Enhancement of Population Size of a Biological Control Agent and Efficacy in Control of Bacterial Speck of Tomato through Salicylate and Ammonium Sulfate Amendments. Appl. Environ. Microbiol. 69: 1290-1294 [Abstract] [Full Text]  
• Klemba, M., Jakobs, B., Wittich, R.-M., Pieper, D. (2000). Chromosomal Integration of tcb Chlorocatechol Degradation Pathway Genes as a Means of Expanding the Growth Substrate Range of Bacteria To Include Haloaromatics. Appl. Environ. Microbiol. 66: 3255-3261 [Abstract] [Full Text]  
• Bastiaens, L., Springael, D., Wattiau, P., Harms, H., deWachter, R., Verachtert, H., Diels, L. (2000). Isolation of Adherent Polycyclic Aromatic Hydrocarbon (PAH)-Degrading Bacteria Using PAH-Sorbing Carriers. Appl. Environ. Microbiol. 66: 1834-1843 [Abstract] [Full Text]  
• Mesarch, M. B., Nakatsu, C. H., Nies, L. (2000). Development of Catechol 2,3-Dioxygenase-Specific Primers for Monitoring Bioremediation by Competitive Quantitative PCR. Appl. Environ. Microbiol. 66: 678-683 [Abstract] [Full Text]  
• Grimm, A. C., Harwood, C. S. (1999). NahY, a Catabolic Plasmid-Encoded Receptor Required for Chemotaxis of Pseudomonas putida to the Aromatic Hydrocarbon Naphthalene. J. Bacteriol. 181: 3310-3316 [Abstract] [Full Text]  
• Tsoi, T. V., Plotnikova, E. G., Cole, J. R., Guerin, W. F., Bagdasarian, M., Tiedje, J. M. (1999). Cloning, Expression, and Nucleotide Sequence of the Pseudomonas aeruginosa 142 ohb Genes Coding for Oxygenolytic ortho Dehalogenation of Halobenzoates. Appl. Environ. Microbiol. 65: 2151-2162 [Abstract] [Full Text]  
• Barriault, D., Durand, J., Maaroufi, H., Eltis, L. D., Sylvestre, M. (1998). Degradation of Polychlorinated Biphenyl Metabolites by Naphthalene-Catabolizing Enzymes. Appl. Environ. Microbiol. 64: 4637-4642 [Abstract] [Full Text]  
• Stuart-Keil, K. G., Hohnstock, A. M., Drees, K. P., Herrick, J. B., Madsen, E. L. (1998). Plasmids Responsible for Horizontal Transfer of Naphthalene Catabolism Genes between Bacteria at a Coal Tar-Contaminated Site Are Homologous to pDTG1 from Pseudomonas putida NCIB 9816-4. Appl. Environ. Microbiol. 64: 3633-3640 [Abstract] [Full Text]  
• Berardesco, G., Dyhrman, S., Gallagher, E., Shiaris, M. P. (1998). Spatial and Temporal Variation of Phenanthrene-Degrading Bacteria in Intertidal Sediments. Appl. Environ. Microbiol. 64: 2560-2565 [Abstract] [Full Text]  
• Fuenmayor, S. L., Wild, M., Boyes, A. L., Williams, P. A. (1998). A Gene Cluster Encoding Steps in Conversion of Naphthalene to Gentisate in Pseudomonas sp. Strain U2. J. Bacteriol. 180: 2522-2530 [Abstract] [Full Text]