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Respiratory Development in Saccharomyces cerevisiae Grown at Controlled Oxygen Tension

¹ Department of Developmental Biology, Research School of Biological Sciences, Australian National University, Canberra, 2601, Australia

ABSTRACT

Saccharomyces cerevisiae was grown in batch culture over a wide range of oxygen concentrations, varying from the anaerobic condition to a maximal dissolved oxygen concentration of 3.5 µM. The development of cells was assayed by measuring amounts of the aerobic cytochromes aa₃, b, c, and c₁, the cellular content of unsaturated fatty acids and ergosterol, and the activity of respiratory enzyme complexes. The half-maximal levels of membrane-bound cytochromes aa₃, b, and c₁, were reached in cells grown in O₂ concentrations around 0.1 µM; this was similar to the oxygen concentration required for half-maximal levels of unsaturated fatty acid and sterol. However, the synthesis of ubiquinone and cytochrome c and the increase in fumarase activity were essentially linear functions of the dissolved oxygen concentration up to 3.5 µM oxygen. The synthesis of the succinate dehydrogenase, succinate cytochrome c reductase, and cytochrome c oxidase complexes showed different responses to changes in O₂ concentration in the growth medium. Cyanide-insensitive respiration and P₄₅₀ cytochrome content were maximal at 0.25 µM oxygen and declined in both more anaerobic and aerobic conditions. Cytochrome c peroxidase and catalase activities in cell-free homogenates were high in all but the most strictly anaerobic cells.

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