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Genetic Analysis of the System that Reduces Biotin-d-Sulfoxide in Escherichia coli

^a Department of Biological Sciences, Stanford University, Stanford, California 94305

ABSTRACT

Four genes of Escherichia coli whose products are needed to reduce biotin-d-sulfoxide to biotin have been mapped: bisA next to chlA, bisB next to chlE, bisC linked to xyl, and bisD next to chlG. A defective transducing phage, dbis5, which carries all the bacterial genes between the attachment site and chlE, was isolated and shown to have lost the phage genes from int through Q.

¹ Present address: Department of Genetics, Research School of Biological Sciences, P.O. Box 475, Canberra City, A.C.T. 2601, Australia.